Journal: Gut Pathogens

Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

doi: 10.1186/s13099-025-00770-9

Figure Lengend Snippet: Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 2. ( A ) Frequencies of IL-17 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( B ) Frequencies of IL-4 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( C ) Frequencies of IL-10 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: The enriched cell fractions were fixed with eBioscienceTM, FoxP3 staining buffer (Thermo Fisher Scientific, Waltham, MA USA), and subsequently intracellularly stained ( Panel 1 : APC anti-human IFNγ; APC/Cy7 anti-human IL-2; PerCP/Cy5.5 anti-human TNFα; all from BioLegend, Koblenz, Germany; FITC anti-human CD154 from Miltenyi Biotec, Bergisch Gladbach, Germany; Panel 2 : PerCP/Cy5.5 anti-human IL-4; APC anti-human IL-10; APC/Cy7 anti-human IL-17a all from BioLegend; FITC anti-human CD154 from Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques:

Journal: Scientific Reports

Article Title: Investigation of an oncolytic herpes simplex virus as a potential therapeutic agent for gastroenteropancreatic neuroendocrine neoplasms

doi: 10.1038/s41598-025-98588-7

Figure Lengend Snippet: QGP-1 and COA109 cells express HSV entry receptors. ( A ) The cell surface expression of viral entry receptors for M002 (CD111, CD112, syndecan, and HVEM) was assessed utilizing flow cytometry. QGP-1 (10 6 ) or COA109 (10 6 ) cells were cultured overnight in 6-well plates, collected, stained with fluorescent conjugated antibody, and evaluated with flow cytometry. Cells treated with FcR blocker alone served as negative controls. Receptors were expressed in variable quantities, but all four receptors were present on both cell types. ( B ) Representative histograms of flow cytometry for viral entry receptors in QGP-1 cells. Data represent three biologic replicates and are reported as mean ± standard error of the mean (SEM). * p ≤ 0.05, ** p ≤ 0.01, COA109 vs QGP-1.

Article Snippet: Briefly, phycoerythrin (PE) conjugated anti-human CD111 antibody (10 μL, Miltenyi Biotec), PE anti-human CD112 antibody (10 μL, Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (10 μL, Miltenyi Biotec), or APC anti-human HVEM (10 μL, Miltenyi Biotec), were added for 20 min on ice in the dark.

Techniques: Expressing, Flow Cytometry, Cell Culture, Staining